Morphometric traits of the heart in standard and mutational colour variants of American mink ( Neovison vison ) / Características morfométricas del corazón en las variantes de color estándar y mutacional del visón americano ( Neovison vison )

The aim of this study was to estimate the values of morphological traits of myocardium in American minks. The study was conducted on 342 male mink hearts and 416 female mink hearts. Mink coat coloration resulting from mutation or crossbreeding of mutational variants with each other and sex were assumed as a source of variation. Carcass, lung and heart weights, heart height, width, depth and circumference, as well as left and right ventricular wall weights and thickness at two locations were determined. The values of 10 indices characterising the relative size of the heart were estimated. The results showed no normal distribution of the heart traits examined. The greatest average heart weight was characteristic of male mutational colour variant minks (17.40 ± 2.34 g). These hearts were heavier by more than 8 % than those of male standard colour variant minks. The hearts of male mutational colour variant minks were characterised by the greatest left and right ventricle weights (P≤0.01) compared to those of male standard colour variant minks, in which in turn the greatest left and right ventricle wall thickness was larger than that in standard colour variant minks. It was found that a greater difference calculated between mean left ventricle wall thickness and mean right ventricle wall thickness in standard colour variant minks may provide more evidence of its adaptation to a greater effort, referring thus to their evolutionary history than to the occurrence of signs of multistage myocardial hypertrophy.

El objetivo de este estudio fue estimar los valores de los rasgos morfológicos del miocardio en el visón americano. El estudio se realizó en 342 corazones de visón macho y 416 corazones de visón hembra. La coloración de la capa de visón resultante de la mutación o el cruce de variantes mutacionales entre sí, y el sexo se asumieron como una fuente de variación. Se determinaron los pesos de la canal, los pulmones y el corazón, la altura del corazón, el ancho, la profundidad y la circunferencia, así como los pesos y el grosor de las paredes de los ventrículos izquierdo y derecho en dos ubicaciones. Se estimaron los valores de 10 índices que caracterizan el tamaño relativo del corazón. Los resultados no mostraron una distribución normal de los rasgos de los corazones examinados. El mayor peso promedio del corazón fue característico de los visones de variante de color mutacional macho (17,40 ± 2,34 g). Estos corazones eran más pesados en más de un 8 % que los de los visones con variante de color estándar machos. Los corazones de los visones de variante de color mutacional macho se caracterizaron por los mayores pesos de los ventrículos izquierdo y derecho (P≤0,01) en comparación con los de los visones de color estándar machos, en los que a su vez el mayor grosor de las paredes de los ventrículos izquierdo y derecho fue mayor que el de las variantes de colores estándar. Se observó que una mayor diferencia entre los grosores medio de las paredes de los ventrículos izquierdo y derecho en las variantes de color estándar, puede proporcionar más pruebas de su adaptación a un mayor esfuerzo, refiriéndose así a su historial evolutivo, pese a la aparición de signos de hipertrofia miocárdica multietapa.

Detection of Multi-drug Resistant Acinetobacter Lwoffii Isolated from Soil of Mink Farm / 生物医学与环境科学（英文）

There were 4 Acinetobacter lwoffii obtained from soil samples. The antimicrobial susceptibility of the strains to 16 antimicrobial agents was investigated using K-B method. Three isolates showed the multi-drug resistance. The presence of resistance genes and integrons was determined using PCR. The aadA1, aac(3')-IIc, aph(3')-VII, aac(6')-Ib, sul2, cat2, floR, and tet(K) genes were detected, respectively. Three class 1 integrons were obtained. The arr-3-aacA4 and blaPSE-1 gene cassette, which cause resistance to aminoglycoside and beta-lactamase antibiotics. Our results reported the detection of multi-drug resistant and carried resistant genes Acinetobacter lwoffii from soil. The findings suggested that we should pay close attention to the prevalence of multi-drug resistant bacterial species of environment.

Research into the antibody detection technology of mink plasmacytosis and its current applications / 病毒学报

Mink plasmacytosis, caused by Aleutian Mink Disease Virus (AMDV), poses a threat to the development of the animal fur industry. Neutralizing antibodies against AMDV may result in a persistent infection rather than providing protection for minks. To date,no specific methods to prevent or cure this disease have been developed. In order to eliminate mink plasmacytosis, antibody detection technology has been used globally as a dominant approach to screen for AMDV-positive minks. This paper introduces the classical technology, counterimmunoelectrophoresis and emerging technology in terms of AMDV antibody detection,and provides a glimpse into the future development of these technologies.

Sequencing Analyses of the Hypervariable Region within the VP2 Gene of a Strain of the Aleutian Mink Disease Virus / 病毒学报

To analyze the molecular mechanisms of cross-host transmission of the Aleutian mink disease vi rus (ADV), the hypervariable region fragment of the VP2 gene of the ADV in Jilin Province (China) was amplified. Sequencing analyses showed diversity at residue 174 by comparison with other VP2 genes in GenBank. The phylogenetic tree indicated that the ADV-JL strain had a close relationship with the highly pathogenic strain from Denmark: ADV-K. Results implied that residue 174 may be associated with ADV infectivity.

An attempt to determine the size of biometric differences in the skull of two colour variants of American mink (Neovison vison) / Un intento de determinar la magnitud de las diferencias biométricas del cráneo de dos variedades de color del visón americano (Neovison vison)

This study aimed at answering the question whether production of new colour variants of American mink in mink farms using mutations may entail changes in skull morphology and relationships between the bone elements building it. Analyses were made on the skulls of 56 eight-month-old males and females of two American mink colour variants (standard Brown and mutant Sapphire) from the same farm. Mean values, standard deviations and coefficients of variation were determined for carcass weight, cranial and mandibular weights and 7 dorsal surface, 8 lateral surface and 11 basal surface traits of the skull. The values of 24 cranial and mandibular indices and the values of sexual size dimorphism (SSD), i.e. a coefficient describing differences between sexes, were calculated. It was demonstrated that mutant colour variants of American mink may be a significant source of variation (P0.05 and P0.01) for some traits of skull morphology and relationships between respective bone elements of viscerocranium and neurocranium.

El objetivo de este estudio fue responder a la pregunta de si la producción de nuevas variedades de color del visón americano en granjas mediante mutaciones puede causar cambios en la morfología del esqueleto de la cabeza y en las relaciones mutuas de los elementos óseos que lo construyen. Los estudios se realizaron en 56 machos y hembras de ocho meses de dos variedades de color del visón americano (bronce estándar y zafiro por mutación) derivados de la misma granja. Se determinaron valores medios, DE y coeficiente de variación para peso corporal, del cráneo y la mandíbula, además de 7 rasgos de la superficie del dorso, 8 de la superficie lateral y 11 características de la base del cráneo. Fueron calculados los valores de 24 índices craneales y mandibulares, junto al valor de la magnitud del dimorfismo sexual, i.e. un coeficiente de las diferencias entre los sexos. Se demostró que las variedades mutantes de color del visón pueden ser fuente significativa (p0,05 y p0,01) de variación para algunas características morfológicas del esqueleto de la cabeza y relaciones entre elementos óseos del víscero y neurocráneo.

Eenie, Meenie, Miney, Moe, who is responsible for the antibody-dependent enhancement of Aleutian mink disease parvovirus infection? / 病毒学报

Aleutian mink disease parvovirus (AMDV) causes a persistent infection associated with immune complex disease, hypergammaglobulinemia, and high levels of antiviral antibodies. Despite the presence of an antibody, the virus is not cleared in vivo. Pre-existing antibodies may enhance viral infections, by Fc-receptor-mediated antibody-dependent enhancement (ADE), but the mechanism that underlies ADE has not been fully defined. Three models have been proposed, including: (1) interactions between antibody and FcR, complement C3 fragment and CR, or between C1q and C1qR, which promotes viral attachment to cells; (2) suppression of IFN-gamma-mediated host-cell antiviral gene expression by the upregulation of negative regulators of pathogen pattern recognition; and (3) the promotion of early IL-10 secretion. In addition, the role of cytokine IL-6 in ADE mediated disease development is discussed, to facilitate a better understanding of the pathogenesis of AMDV infection, as well as give insights into rational vaccine design approaches.

Nuclear factor E2-related factor 2 Dependent Overexpression of Sulfiredoxin and Peroxiredoxin III in Human Lung Cancer

BACKGROUND/AIMS: Oxidative stress results in protein oxidation and is implicated in carcinogenesis. Sulfiredoxin (Srx) is responsible for the enzymatic reversal of inactivated peroxiredoxin (Prx). Nuclear factor E2-related factor 2 (Nrf2) binds to antioxidant responsive elements and upregulates the expression of Srx and Prx during oxidative stress. We aimed to elucidate the biological functions and potential roles of Srx in lung cancer. METHODS: To study the roles of Srx and Prx III in lung cancer, we compared the protein levels of Nrf2, Prxs, thioredoxin, and Srx in 40 surgically resected human lung cancer tissues using immunoblot and immunohistochemical analyses. Transforming growth factor-beta1, tumor necrosis factor-alpha, and camptothecin treatment were used to examine Prx III inactivation in Mv1Lu mink lung epithelial cells and A549 lung cancer cells. RESULTS: Prx I and Prx III proteins were markedly overexpressed in lung cancer tissues. A significant increase in the oxidized form of a cysteine sulfhydryl at the catalytic site of Prxs was found in carcinogenic lung tissue compared to normal lung tissue. Densitometric analyses of immunoblot data revealed significant Srx expression, which was higher in squamous cell carcinoma tissue (60%, 12/20) than in adenocarcinoma (20%, 4/20). Also, Nrf2 was present in the nuclear compartment of cancer cells. CONCLUSIONS: Srx and Prx III proteins were markedly overexpressed in human squamous cell carcinoma, suggesting that these proteins may play a protective role against oxidative injury and compensate for the high rate of mitochondrial metabolism in lung cancer.

Chordoma in the Tail of a Ferret / 한국실험동물학회지

A chordoma is an uncommon tumor that originates from the remnants of the notochord and most commonly involves the cranial and caudal regions of the axial skeleton. Chordoma has been described in laboratory animals such as dogs, rats, minks, and ferrets. This report describes a case of a chordoma in the tail of a ferret. Grossly, a grayish-white, expansile, subcutaneous soft-tissue mass was observed in the tail. Histopathologically, the mass was a loosely placed, nodular, unencapsulated neoplasm within the dermis. In the mass, tumor lobules were intermingled with fibrous tissues. Fibrous tissues contained abundant extracellular basophilic material that was consistent with mucin. The tumor was composed of a close pack of adipocyte-like vacuolated cells (physaliferous cells). The cells were centrally or eccentrically located round nuclei and eosinophilic cytoplasm with large vacuoles. Immunohistologically, neoplastic cells were positive for vimentin and S-100 protein. Based on histopathologic findings and special staining characteristics, this case was diagnosed as chordoma.

Effect of gingerol on substance P and NK1 receptor expression in a vomiting model of mink / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Gingerol is the generic term for pungent constituents in ginger, which has been reported to be effective for inhibiting vomiting. We attempted to investigate the antiemetic effect of gingerol and its effective mechanism on substance P and NK(1) receptors in minks.</p><p><b>METHODS</b>The antiemetic effect of gingerol was investigated during a 6-hour observation on a vomiting model in minks induced by cisplatin, (7.5 mg/kg, intraperitoneal). The distribution of substance P and NK(1) receptors in the area postrema and ileum were measured by immunohistochemistry, and the expression of NK(1) receptor in the area postrema and ileum were measured by Western blotting.</p><p><b>RESULTS</b>The frequency of cisplatin induced retching and vomiting was significantly reduced by pretreatment with gingerol in a dose-dependent manner (P < 0.05). Substance P-immunoreactive was mainly situated in the mucosa and submucosa of the ileum as well as in the neurons of the area postrema. The immunoreactive production of NK(1) receptor was mainly situated in the muscular and submucosa of ileum and the neurons of area postrema, gingerol markedly suppressed the increased immunoreactivity of substance P and NK(1)1 receptor induced by cisplatin in a dose-dependent manner (P < 0.05), and exhibited effective inhibition on the increased expression levels of NK(1) receptor in both the ileum and area postrema dose-dependently (P < 0.05).</p><p><b>CONCLUSIONS</b>Gingerol has good activity against cisplatin-induced emesis in minks possibly by inhibiting central or peripheral increase of substance P and NK(1) receptors.</p>

RAPD genetic analysis on etiological factor of mink self-biting disease / 生物工程学报

Self-biting is a chronic disease, which cause wound to take effect on mink growth and pelt quality. In this study, we firstly adopted RAPD (random amplification polymorphism DNA) technique based on the reproducible 26 polymorphism primers screened from 100 random primers to analyze hereditary constitution of the samples from healthy minks and self-biting minks, respectively, at molecular level to aim to discuss the causes of self-biting. The results showed that 29 straps showed polymorphism among amplified 105 straps, of which the polymorphism rate is 27.62%. Between healthy and sick mink groups, the amplified DNA fragment through different primers indicated different distribution frequency. The similarity coefficient of mink groups is 0.8471 and genetic distance (variation) index is 0.1529. Through primer S356 (whose sequence is CTGCTTAGGG), we amplified different straps between healthy and sick mink. The amplified 1000 bp DNA fragment in the sick mink groups can preliminarily serve as molecular genetic label to distinguish from healthy and sick mink groups to gradually remove the mink individual of self-biting, achieve to purify mink groups and reduce economy loss of mink breeding industry. This work provide theoretical basis for further study on molecular breeding and disease prevention of mink.

Effect of Rhizoma pinelliae on vomiting in minks / 中国中药杂志

<p><b>OBJECTIVE</b>To study emetic and anti-emetic effects of Rhizoma pinelliae in minks.</p><p><b>METHOD</b>The emetic effect of raw pinellia 2 g kg(-1) (i.g.) was investigated. Three preparations of Rhizoma pinelliae (processed with ginger) were made by ethanol extraction, water extraction and water decoction respectively and their effects on emesis model induced by cisplatin (7.5 mg kg(-1), i.p.) or apomorphine (1.6 mg kg(-1), s.c.) were then studied; the effect of the decoction of ginger-processed Rhizoma pinelliae on rotation-induced emesis model in minks was also observed.</p><p><b>RESULT</b>The emesis was induced by raw pinellia in minks (P < 0.01); ginger-processed Rhizoma pinelliae, metoclopramide and ondansetron significantly inhibit the emesis induced by cisplatin and apomorphine (P < 0.05).</p><p><b>CONCLUSION</b>Ginger-processed Rhizoma pinelliae exhibits a anti-emetic effect in minks, which may be mediated by inhibiting the function of the vomiting center in central nervous system.</p>

Mechanisms of sodium ferulate inhibition of collagen synthesis in hepatic stellate cells / 药学学报

<p><b>AIM</b>To study the mechanisms of sodium ferulate (SF) on inhibition of collagen synthesis in hepatic stellate cells.</p><p><b>METHODS</b>Collagen synthesis was analyzed by measuring 3H-proline incorporation. ELISA method was used to study the effect of SF on transforming growth factor beta1 (TGFbeta1) level in cultured HSC-T6 cell. The effect of SFon the TGFbeta1 activity in the supernatant of culture was analyzed by mink lung epithelial cell (Mv1Lu) proliferation inhibition by MTT assay.</p><p><b>RESULTS</b>SF inhibited collagen synthesis in hepatic stellate cells stimulated with TGFbeta1. SF was shown to decrease TGFbeta1 level in the supernatant of HSC-T6 increased by oxidative stress. TGFbeta1 activity was intervened by SF.</p><p><b>CONCLUSION</b>SF could decrease collagen synthesis, with mechanism may be associated with that SF intervened TGFbeta1 activity, and reduced the level of TGFbeta1 increased by oxidative stress.</p>

Effects of rhein on the function of human mesangial cells in high glucose environment / 药学学报

<p><b>AIM</b>To study the mechanisms of anti-diabetic nephropathy of rhein on cultured human mesangial cells (HMCs).</p><p><b>METHODS</b>To mimic the hyperglycemic (HG) environment of diabetic nephropathy, 30 mmol x L(-1) glucose were added to 10% FBS RPMI 1640. The HMCs were treated with rhein for 8, 24, 48 or 72 h, at these time, the bioactivity, total activity of transforming growth factor-beta1 (TGFbeta1), activity of p38MAPK (p38 mitogen-activated protein kinases, by using immunoprecipitate and Western blot), MMP-2 (matrix metalloproteinase-2), and MMP-9 (matrix metalloproteinase-9, by using gelatinase zymography) and the proliferation of HMCs in high glucose media were measured. Meanwhile the levels of secretion of FN in cultured HMCs were measured.</p><p><b>RESULTS</b>The results showed that rhein markedly inhibit the proliferation of HMCs, significantly reduce the bioactivity of TGFbeta1 and FN secretion in HMCs, and decrease the increased activity of p38MAPK, but showed no action on the activities of MMP-2 and MMP-9.</p><p><b>CONCLUSION</b>Rhein reduced the secretion of FN and inhibited the proliferation of HMCs may through inhibiting the bioactivities of TGFbeta1 and p38MAPK.</p>

A new vomiting animal model--mink / 药学学报

<p><b>AIM</b>To establish a new, reliable vomiting model in minks.</p><p><b>METHODS</b>Adult male minks (Mustela vison) were randomly divided into groups (n = 6). Cisplatin, apomorphine, copper sulfate and X-radiation were used to establish vomiting model. Retching and vomiting were observed after the vomiting models were given anti-vomiting agents. After the behavioral experiment, assay of 5-HT in the ileum was performed by immunohistologic method.</p><p><b>RESULTS</b>Cisplatin 7.5 mg.kg-1 i.p., apomorphine 1.6 mg.kg-1 s.c. and copper sulfate 40 mg.kg-1 ig were shown to evoke vomiting. Retching and vomiting were significantly inhibited in ondansetron and metoclopramide pretreated minks (P < 0.05, P < 0.01).</p><p><b>CONCLUSION</b>As a new vomiting model, minks may be of great value in studying vomiting mechanism and screening new antiemetic drugs.</p>

Growth of respiratory syncytial virus in mink lung epithelial cells.

Mink lung epithelial cells (Mv-1-Lu) were tested for their ability to support the growth and serial passage of respiratory syncytial virus (RSV) in vitro. Indian isolates of RSV induced distinctive cytopathic effect with typical rounding of cells followed by detachment with more than 50 per cent cells showing bright fluorescence using anti-RSV monoclonal antibodies in immunofluorescence test. Serial passage of RSV was possible in Mv-1-Lu cells without loss of sensitivity of the cells for virus growth. Titration of cell associated virus and virus released in the supernatant indicated that 60 per cent of the virus was released in the supernatant, and 40 per cent remained cell associated. Transmission electron microscopic studies of negatively stained RSV particles and ultra-thin sections of RSV infected Mv-1-Lu cells showed roughly spherical particles with club shaped projections, budding from the cytoplasmic membrane. These results indicate that Mv-1-Lu cell line is suitable for the growth and propagation of RSV.

Localization of the connexin 43 gap-junction protein in silica-exposed alveolar epithelial cells / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the effect of supernatants from silicon dioxide(SiO2) stimulated pulmonary alveolar macrophages(PAM) on the localization of connexin 43(Cx43) so as to explore the inhibition level of SiO2 on alveolar epithelial cellular gap-junctional communication(GJIC).</p><p><b>METHODS</b>The supermatants from the primary cultured PAM were prepared, and then added 5% (v/v) SiO2 into 2% (v/v) NBS RPMI 1640 to stimulate the normal mink lung epithelial cell line CCL-64 for 24 hours. The localizations of Cx43 in CCL-64 were analyzed by indirect immunofluorescence histochemistry and laser confocal scanning microscopy(LCSM).</p><p><b>RESULTS</b>The normal cultured CCL-64 cells displayed bright membrane-associated Cx43 plaques labeling and formed dashes at regions of intercellular junction. Being exposed to supernatants from SiO2-stimulated PAM, the CCL-64 cells retained a relative low degree of Cx43 labeling at the cell periphery, localized in cytoplasm, and the individual spot, rather than plaques, were smaller compared to normal cultured cells. Along with the increase of the concentrations of SiO2, the cells displayed a different staining pattern, with clear cluster labeling aggregating towards the nucleus.</p><p><b>CONCLUSION</b>The altered localization of the gap-junctional protein Cx43 in alveolar epithelial cells, mediated by SiO2, indicated that the internalization of Cx43 may contribute to the inhibition on GJIC in silica-induced lung epithelium injury.</p>

Changes of phosphorylation states of Cx43 protein in CCL-64 cells induced by SiO2 / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To investigate whether the cellular gap junctional communication(GJIC) down-regulation in alveolar epithelial cells (CCL-64 cells) induced by silica-stimulated pulmonary alveolar macrophages (PAM) supernatant is related with the phosphorylation states of connexin 43(Cx43) protein.</p><p><b>METHOD</b>Western-blot analysis was used to identify phosphorylated Cx43 species.</p><p><b>RESULTS</b>Western-blot analyses of SiO2- and phorbol 12-myristate 13-acetate(TPA)-treated CCL-64 cells showed the same phosphorylation states of Cx43 as the control group. There were no Cx43 protein in nucleus of CCL-64 cells.</p><p><b>CONCLUSION</b>The inhibition on GJIC induced by SiO2 and TPA in CCL-64 cells may not be brought about by altering the phosphorylation states of Cx43.</p>

Purification and Characterization of Transforming growth factor - beta1 from Human Platelets / 대한면역학회지

Transforming growth factor-j31 (TGF-p1) has potential for therapeutic use in common clinical conditions for which there are no adequate pharmacological agents. However, in vivo studies using TGF-p1 were hindered by high price of this cytokine. As a first step towards large scale purification of TGF-p1, it was purified in a small scale (10 unit platelets) from human platelets by four purification steps: platelet extraction, gel filtration, cation exchange chromatography, and reversed phase high performance liquid chromatography (HPLC). A single protein band with a molecular weight of 25 Kd corresponding to purchased TGF-p1 (R8D Systems) was confirmed by silver staining after SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of eluant from reversed phase HPLC. Recovery (%) of each step was about 50-60%, resulting in the final recovery of 20% based on the detection by a sandwich ELISA. Approximately, 3.7 p,g of purified TGF-p1 was obtained from 18 pg of platelet extracts. This result was confirmed by receptor (TGF-j31 type II) ELISA and bioassay using a mink lung epithelial'cell line (MV1LU). Further, in vitro characterization study showed that purified TGF-p1 inhibits G1/S transition of LPS-activated murine spleen B cells and increases surface IgA expression by the same cell population, which are typical activities of TGF-p1 in B cell differentiation. Taken together, the results from the present study reveals that purified TGF-p1 is fully biologically active and our purification methodology could be usbful to obtain a large scale of recombinant TGF-p1 in the future.

Efecto del carcinogeno dimetilnitrosamina sobre el rinon del vison / Efect of carcinogen dimethylnitrasamine on the kidney of mink

Visones de un criadero que recibian alimentos, sobre la base de restos de pescado, evidenciaron un significativo aumento en su mortalidad, presencia de canceres hepaticos y alteraciones renales revelables histologicamente. Esos efectos fueron atribuibles a presencia, en el alimento, de dimetilnitrosamina (NDMA), en concentraciones 1,8 ug/g. En este trabajo se estudia en detalle el efecto de la NDMA sobre el rinon del vison. Visones que fueron tratados ip con NDMA(7 mg/kg en sol. fis.), mostraron dano evidenciable ultraestructuralmente en la corteza renal. El dano fue mayor en los tubulos proximales, que en los distales, pero era de naturaleza similar. Las celulas epiteliales tubulares de los animales intoxicados mostraron: a)Condensacion de la cromatina nuclear y dilatacion de la membrana perinuclear. b)Marcada hinchazon mitocondrial y ruptura de sus crestas con perdida de contenida de la matriz mitocondrial. c)Despegue de ribosomas y dilatacion del reticulo endoplasmico. d)Aumento del numero y tamano de las vacuolas autofagicas. e)Aparicion de gotas lipidicas en el citiplasma. En contraste con lo previamente establecido, para el caso de cancer hepatico del vison, el mecanismo del dano renal por NDMA no se pudo correlacionar directamente con la union de metabolitos reactivos de esta a proteinas o acidos nucleicos o la biotransformacion microsomal o mitocondrial de la NDMA o formaldehido. No obstante, el rinon biotransforma la NDMA a CO2, pero lo hace 3-4 veces menos intensamente que el rinon de rata. Los resultados sugeririan la presencia, en el caso del dano renal por NDMA, de mecanismos distintos de accion, a los habitualmente aceptados como responsables del dano hepatico o el renal en otras especies. Alternativamente, el dano renal puede deberse a dano hepatico concomitante

Neumonía hemorrágica por Pseudomonas aeruginosa en visones de la República Argentina / Hemorrhagic pneumonia by Pseudomonas aeruginosa in minks of Argentina

Esta es la primera comunicación acerca de la existencia de casos de neumonía hemorrágica en visones en la República Argentina y en el hemisferio sur. La epidemiología de la enfermedad es similar a la descripta en otros países, al igual que la sintomalogía y las lesiones macro y microscópicas. El diagnóstico se efectuó con el aislamiento de Pseudomonas aeruginosa en repetidas ocasiones de los pulmones afectados, siendo el serotipo nro. 6 (Sistema Difco) el más frecuente. Se reprodujo experimentalmente la enfermedad infectando visones por vía intranasal previa narcosis de los mismos